Helix server fiu11/11/2022 Thus, in this study, we tried to isolate such a high copy number shuttle vector derived from the pHM1519-replicon. However, shuttle vectors with high copy number are required for some protein expression in C. glutamicum strains had been estimated to be around 40 copies per cell (unpublished result). coli shuttle vector pPK4 provides kanamycin tolerance to both host strains, and it is useful for DNA manipulation in C. Using pHM1519, a variety of shuttle vectors containing another replicon driven in Escherichia coli have been constructed, a representative of which is pPK4. pCG1- and pCGR2-derived mutant shuttle plasmids exhibiting about 20–30 copies per chromosome were constructed by weakening the promoter activity of the small antisense RNA transcript. They characterized a small RNA encoded in the antisense strand sequence of DNA encoding an initiator Rep protein and indicated that the antisense RNA negatively controls plasmid replication through an interaction between the small RNA and the mRNA of Rep. reported antisense RNA-mediated plasmid copy number control in pCG1 family plasmids. glutamicum ATCC 13058 was assumed to be around 140 copies in a previous study, but it was reported that the copy numbers of pCG100 and pSR1 are about 30 per chromosome. Plasmids are normally maintained with a defined number of copies in growing populations of bacteria, and plasmid copy number is defined as the ratio of plasmid DNA molecules to chromosomal DNA in the host cell. RepA of pHM1519 is composed of 485 amino acid residues including the conserved Yuxk motif of replication initiator proteins for rolling circle DNA replication. From genetic and molecular analyses of pSR1 and pCG100, it was determined that the autonomous replication region of pHM1519 is located in a 1.9-kbp DNA fragment containing the repA gene, which encodes essential replication protein RepA. In particular the complete nucleotide sequences of pHM1519 (3,070 bp) and pCG1 (3,069 bp, GenBank: AB027714.1) were determined showing only one base difference between them, and pHM1519 is now classified as a member of the pCG1 family of corynebacterial plasmids. glutamicum strains and named pCG1, pSR1, pCG100, and so on. Plasmids very similar to pHM1519 were independently isolated from other C. A small cryptic plasmid pHM1519 was originally found in Corynebacterium glutamicum ATCC 13058.
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Best serial killer documentaries11/10/2022 Night Stalker: The Hunt for a Serial Killer More videos. The docuseries follows pizza masters as they discuss the spin they’ve put on the beloved dish and how it’s shaped their craft, business, and taste. If youre a true crime documentary fan and havent seen these, you need to rectify that immediately. 5) and National Pepperoni Pizza Day (Sept. It’s fitting considering both National Cheese Pizza Day (Sept. The serial killer's crimes spanned from 1960 to 1983 and he was convicted for murdering 11 people. #BEST SERIAL KILLER DOCUMENTARIES SERIES#The 3-part series includes news footage, photos, and interviews with family members of victims, as well as with Todd Kohlhepp himself. Undoubtedly one of the best serial killer documentaries and another Nick Broomfield gem, Tales of the Grim Sleeper was shot with the director’s son Barney Broomfield and Marc Hoeferlin on board as well. Crime thrillers and such are especially good since theyre mysterious (obvious. Tales of the Grim Sleeper (2014) Image credit: Tales of the Grim Sleeper/ IMDb. The five-part American true crime documentary, which is directed by Rovert Kenner and Taki Oldham, is based on the 1983 case of Henry Lee Lucas. One of the best true crime documentaries about a town with a monster in its midst is Investigation Discovery’s Serial Killer: Devil Unchained. Though I believe that killer/murder documentaries are entertaining. They’re grown, out of jail now, and primed to tell their story in all of its wildness.įor our foodies, Chef’s Table has returned with a focus on pizza this go around. The Confession Killer is based on American serial killer Henry Lee Lucas Credit: Netflix. HBO's six-part documentary I'll Be Gone In The Dark adapted the true-crime novel by the late. Serial killer Richard Ramirez terrified the entire city of Los Angeles. If you’d like a lighter true crime doc to add to your watch list, there’s The Real Bling Ring: Hollywood Heist which is centered on the infamous group of teens who invaded the homes of celebrities and stole their possessions. The Best Documentaries About Serial Killers Night Stalker: The Hunt For A Serial Killer. Holmes' entire life and consists of reenactments, authentic locations, period photography, and expert interviews. Holmes, also dubbed the 'Torture Doctor.' Released in 2004, this documentary focuses on Dr. Both series cover murders but the latter documentary involves a cold case that’s opened up due to 13 more victims being found and allegations of cannibalism so be prepared for both the horrific nature of the crime as well as incredibly disturbing elements in the case. Holmes: America's First Serial Killer details the life of America's first serial killer and criminal mastermind, H.H. September is set to bring more exciting titles to Netflix! Much of what’s on offer are scripted titles, but the streamer has a dedicated wing to documentaries as well, so if you’re craving content focused on real people then you won’t be disappointed with this month’s haul.įor true crime buffs, Sins of Our Motherand Indian Predator: Diary of a Serial Killer are likely to be the two titles that will be must-watches. Macross exedore11/10/2022 #MACROSS EXEDORE SERIES#The novel version introduces some interesting ideas that challenged Robotech’s previous boundaries, but overall, it is difficult to find much to praise Robotech II: the Sentinels for in terms of its concepts.īecause they already received a sense of completeness within the Macross series the return of the main Macross characters in The Sentinels feels particularly forced and artificial, all of the sequel stories seem to struggle with what to do with them. It didn’t add any new or compelling facets to most of the returning cast, made a muck of continuity, and the new characters were largely ciphers, with some truly awful character designs and a flimsy backstory. But when compared to anything else, even the original Robotech dub, The Sentinels was bad or at least looked like it could have been. If judged by the standards of the other popular American cartoons of the 1980s, the Sentinels outlines seen about on par. The Sentinels project rapidly fizzled, though it briefly survived in a an animated “pilot” using some of the footage that had been produced, separate comic and novel adaptations, some other released plans and scripts, and scattered references in the eventual rebooted continuity. #MACROSS EXEDORE FREE#This Robotech Expeditionary Force ended up working with a group of local alien rebels (the titular Sentinels) to free their nearby planets from the mutual threat of The Invid (whose other faction formed the villain of the third Robotech segment), as well as dealing with a coup attempt within their ranks. It was originally going to be an animated television “sequel” to Robotech, actually a side story about the Macross characters and their journey to Tirol, the homeworld of the Robotech Masters (characters from the second segment). The big beast of the Robotech apocrypha was a1986 project called Robotech II: The Sentinels. I soon realized that everything was largely a garbage pile that few sane fans would bother with, but even after realizing that, I still stuck with the Robotech EM because of those very small things related to the Zentraedi. Initially I devoured almost everything in the Robotech EM without thought to quality or consequence, initially distracted by the shine of works that actually thought about my favourite characters and offered them further growth. #MACROSS EXEDORE CRACK#Yet since the material still existed, and contained some Zentraedi stories, I wanted to take a crack at it. Macross is good for the Zentraedi as a whole group, but the specific approach to certain characters leaves something to be desired, and in Robotech (at least, the old novels and comics) is good for some certain Zentraedi, but the general handling of the race is godawful.īy the time I came on the scene, there had already been scads of Robotech comics and a novel series published, and all long out of print and recently declared “not canon”. I experienced both sides more or less simultaneously, and in the end, I couldn't discard either of them for the sake of my interest in Zentraedi. Instead, many strange things happened, and matters instead branched off into two basic forks: the Robotech expanded multiverse (most of it currently deemed unofficial) and the mainstream Macross continuity. To learn more, see the privacy policy.You would think, if my focus were primarily on a Macross element, I would have nothing to do with Robotech after my initial probing. Please note that Related Words uses third party scripts (such as Google Analytics and advertisements) which use cookies. #MACROSS EXEDORE CODE#Special thanks to the contributors of the open-source code that was used to bring you this list of term themed words: Concept Net, WordNet, and is still lots of work to be done to get this to give consistently good results, but I think it's at the stage where it could be useful to people, which is why I released it. You will probably get some weird results every now and then - that's just the nature of the engine in its current state. related words - rather than just direct synonyms.Īs well as finding words related to other words, you can enter phrases and it should give you related words and phrases, so long as the phrase/sentence you entered isn't too long. These algorithms, and several more, are what allows Related Words to give you. 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The 2014 is not an obvious Opus One, like the 2010, 2012 or 2013, but over time, I will not be surprised if it challenges or even surpasses some of those vintages. A sleeper wine for the year, the 2014 is shaping up to be a gorgeous wine that will handsomely repay several decades of cellaring. The tannins are firm but also impeccably balanced. Savory, tightly wound and intensely aromatic, the 2014 is going to need time to unwind, but it looks like it will develop along the more classic vintages here. "The 2014 Opus One has turned out to be a gorgeous wine. Exquisite tension and finesse with an almost Burgundian texture, yet it’s Napa in its soul with ripe fruit, stones, spices and Chinese tea leaf flavors. "Mesmerizing aromas of flowers, bark, currants and blackberries. Postbox app11/9/2022 The developers are also very responsive, and the app continues to get better at a rapid clip. My personal favorite is Domain Fencing, which makes sure that you don’t accidentally send a work email from your personal account (or vice versa). For example, the first Labs project announced will bring OpenPGP support to Postbox, allowing you to encrypt the email that you send.Īll of this is in addition to an already incredibly powerful email client with several unique features, like one-click unsubscribe, Pre-Made responses (like Canned Responses in Gmail), and Quick Post (which allows you to pre-configure the email send-to services that many popular productivity apps use). It’ll be interesting to see developer support for this since Postbox won’t support Lab features directly, but it does open up interesting possibilities. Unlike the Add-Ons which are a simple installation process, Labs is literally bolting on additional third-party code. POSTBOX APP CODEThere’s also a new Labs feature that allows custom code to be added to Postbox that extends the functionality of the app. The Clips library includes everything from custom bullets to callouts and tables, and can be inserted wherever you want the element to appear in your email. So in version 7, Postbox gives you a library of pre-made “clips” that make this a lot easier. This allowed you to create newsletters, brand templates, or company email stationery, but many people don’t want to mess with pure HTML if they don’t have to. For example, one of the unique features that Postbox sported previously was the ability to fully customize and send HTML email from within the app. Make no mistake, Postbox is still a very powerful email client. All 20 themes also change the appearance across the entire app, as they also include message preview and compose windows. I personally like the Dark theme, as it fits in very well with the Dark Mode on macOS Mojave or later. The themes range from very light to very dark, but they all look really good. I’m usually pretty picky about the themes I use in my apps, and often find only one or two that are usable and don’t drive me crazy. Postbox 7 also got a facelift and now offers 20 themes to choose from. You can tell the team put a lot of work into the polish of the app, and there are lots of little things that have been tightened up in the app so that it just feels better. There’s a lot to like in this new version, but the thing that stands out to me after using it for about a week is just how much more solid everything feels. POSTBOX APP MACThere is new clustering functionality in the map interface so that you can better see the information on the map, and a new circular radius overlay that helps you see clearly the area that you are currently viewing postbox data within, since we cannot place all 400,000+ data items on the map at once.Last week, the Postbox team released version 7 of their Mac email client. There is a whole new interface to the app that allows you to request data updates from OpenStreetMap within 16km of the centre of the map, you can now use toggles in the top menu to allow you to filter to only see postboxes, post offices or both. The data is available under the Open Database Licence. We are now using OpenStreetMap at the data source for the postbox and post office location information, “© OpenStreetMap contributors”. POSTBOX APP UPDATEOnce the update is confirmed at OpenStreetMap, you will be able to use the refresh button in the app to download the updates. If you do find missing postboxes, then please help by registering at and creating or updating incorrect information. The coverage is extensive although there are postboxes that are not currently included in the app. Postbox Finder UK helps you locate nearby postboxes and post offices in the UK using the GPS built into your phone. Light level of jack o lantern minecraft11/9/2022 #LIGHT LEVEL OF JACK O LANTERN MINECRAFT HOW TO#Minecraft: How to Craft a Soul Lantern Soul Lantern Minecraft A sea lantern builds a conduit arrangement, along with prismarine brick, prismarine, and dark prismarine. The sea lantern may be used like a”beacon” to help players find their way home after extended explorations. The sea lantern is more robust than a flashlight and operates on property. It’s one of just four blocks that will give off lasting light if it stays underwater, the others being Jack o’ Lanterns, glowstone, and a Redstone lamp. The sea lantern comes with an animated feel and gives a light level of 15. Sea Lantern MinecraftĪ Sea Lantern in Minecraft is a light source that only appears underwater in and around Ocean Monuments. #LIGHT LEVEL OF JACK O LANTERN MINECRAFT UPDATE#Minecraft Lanterns can generate on lamp posts in some types of villages after the upcoming update 1.14. About June 12, 2011, Notch once again hinted he was working on lanterns. But, Notch wouldn’t include torches as a cellular light source because he did not like how they looked. It makes them a useful thing for investigating caves and other dark environments. Minecraft Lanterns could create light while being transported. It is a block that never burns out while lit-having both of these items as components to get a lantern would have needed players to travel to the Nether if they wanted to craft one. A second potential item to make landscapes was a nether rack. That could have made lanterns relatively inaccessible and also a risky item to obtain. And they were hinted by Notch as a possible item requirement to manage the still-unimplemented lanterns since it has the greatest luminescence of almost any raw material. Included in the Halloween Update, glowstone got its introduction. Notch and his Minecraft Lantern Minecraft Lantern Since several years have passed since this announcement, and Notch no longer functions for Mojang, it’s uncertain if this may be the case. Just redesign the present Minecraft torch. It needs to follow Notch, such as in gamers’ stock or at chests. All torches in a world can convert into lanterns. Torches require altering so that they eventually burn out and would have to be re-lit together with flint and steel. At one time, Notch had stated with the reserve of the Lantern. Lanterns is a possible addition to the match for several years, dating back to when Notch was functioning on Minecraft. It gives a mild degree of 15, marginally more than a flashlight. are some simple organic ways to prevent mobs from spawning in low light environments.A lantern in Minecraft can be set on top of or hanging beneath most solid cubes. You can also consider using transparent or partial blocks in areas that are off the main path, so that mobs can't spawn there and you don't need to struggle to find the right lighting. But since torches/jack-o-lanterns are easy to get in bulk, you can place them 3-4 blocks down every 4 blocks or so and your ground should be fully lit. As discussed in the link a couple others have posted, the light level can't be above 12 for any given block, or the snow will melt. Snow! If your base is in an area where snow is appropriate, you can place a torch, jack-o-lantern, or glowstone/sea lantern block a few blocks deep, with a couple glass blocks or your preferred transparent block, and then put snow on top flush with the ground. Another good one is to just stick a fence post one block up from the ground against the wall, and place a torch on top of the post, giving a sort of torch bracket look. If your base has a wall around it, you can use wood/stone detailing and place torches in recessed alcoves along the wall. Then fill in the trench with water.ĭecorative torches: Torches can work well, if you place them in a creative way. and place glowstone in the bottom, so your trench is now one deep (you can do every block, every other, every 4th, etc. Lit waterway: Dig a 1-wide, 2-deep trench on either side of your main pathway, or along your farm, etc. Light poles: One cobble wall, two wooden fence posts, and a glowstone block (optional: place trapdoors on each face of the glowstone for some detailing). AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |